Anti-Human HRAS Recombinant Antibody (Y13-259) (HF778023)

Name: Anti-Human HRAS Recombinant Antibody (Y13-259)
Brand: abinScience
SKU: HF778023
Price: 110 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

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사양:

20ug
50ug
100ug
1mg

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HF778023

종 반응성

Human

응용 분야

IF, IP, WB

숙주 종

Mouse

클론성

Monoclonal

이소타입

IgG2a, kappa

클론 ID

Y13-259

타겟

c-H-ras, GTPase HRas, H-Ras-1, p21ras, Transforming protein p21, Ha-Ras, HRAS1, HRAS

내독소 수준

Please contact with the lab for this information.

순도

>95% as determined by SDS-PAGE.

정제

Protein A/G purified from cell culture supernatant.

등록번호

P01112

RRID

Anti-Human HRAS Antibody (Y13-259) (abinScience Cat# HF778023, RRID:AB_3728964)
_x005F_x005F_x000D_

형태

Liquid

저장 완충액

0.01M PBS pH 7.4

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

배경

GTPase HRas is a ~21 kDa protein. Involved in the activation of Ras protein signal transduction. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

1. Gripp, KW. et al. (2012) American journal of medical genetics. Part A 158A, 2106-18. PMID: 22821884

2. Williams, JG. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 6376-81. PMID: 12740440

3. Guil, S. et al. (2003) Cancer research 63, 5178-87. PMID: 14500341

4. Lander, HM. et al. (1997) The Journal of biological chemistry 272, 4323-6. PMID: 9020151

참고사항

For research use only.

Isotype Control

Mouse IgG2a Recombinant Isotype Control Antibody (S43.10) (MF533307)

이미지

SDS-PAGE

SDS-PAGE for Anti-Human HRAS Antibody (Y13-259).
Flow CytoMetry

Flow-cytometry using FITC anti-human HRAS antibody. MCF-7 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human HRAS monoclonal antibody (Catalog: HF778023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-KRAS/K-Ras 2 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human KRAS/K-Ras 2 (Catalog No: HF904011), 50 ng
Lane 2: recombinant human KRAS/K-Ras 2 (Catalog No: HF904011), 30 ng