Anti-Human IL-2 Polyclonal Antibody (HX011024)

Name: Anti-Human IL-2 Polyclonal Antibody
Brand: abinScience
SKU: HX011024
Price: 168 USD
Availability: InStock

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개요

카탈로그 번호

HX011024

설명

Anti-Human IL2 Polyclonal Antibody (HX011024) is a rabbit polyclonal antibody detecting IL2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human IL2 (Met1-Thr134).

타겟

IL2, IL-2, Aldesleukin, Interleukin-2, TCGF, T-cell growth factor

정제

Purified by antigen affinity column.

등록번호

P60568

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Interleukin-2 (IL2) is a ~17 kDa protein. Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways.

1. Mingari, MC. et al. (1984) Nature 312, 641-3. PMID: 6438535

2. Wang, X. et al. (2005) Science (New York, N.Y.) 310, 1159-63. PMID: 16293754

3. Stauber, DJ. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 2788-93. PMID: 16477002

4. Miyazaki, T. et al. (1994) Science (New York, N.Y.) 266, 1045-7. PMID: 7973659

5. Kirken, RA. et al. (1995) Cytokine 7, 689-700. PMID: 8580378

6. Osinalde, N. et al. (2015) Proteomics 15, 520-31. PMID: 25142963

7. Holbrook, NJ. et al. (1984) Proceedings of the National Academy of Sciences of the United States of America 81, 1634-8. PMID: 6608729

참고사항

For research use only.

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