Anti-Human ITCH Polyclonal Antibody (HP466014)

Name: Anti-Human ITCH Polyclonal Antibody
Brand: abinScience
SKU: HP466014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HP466014

설명

Anti-Human ITCH Polyclonal Antibody (HP466014) is a rabbit polyclonal antibody detecting ITCH in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human ITCH (Met1-Gly141).

타겟

Atrophin-1-interacting protein 4, HECT-type E3 ubiquitin transferase Itchy homolog, NFE2-associated polypeptide 1, NAPP1, E3 ubiquitin-protein ligase Itchy homolog, ITCH, Itch, AIP4

정제

Purified by antigen affinity column.

등록번호

Q96J02

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

E3 ubiquitin-protein ligase Itchy homolog is a ~102 kDa protein. Acts as an Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. Involved in the control of inflammatory signaling pathways. Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of the complex after TNF stimulation.

1. Winberg, G. et al. (2000) Molecular and cellular biology 20, 8526-35. PMID: 11046148

2. Marchese, A. et al. (2003) Developmental cell 5, 709-22. PMID: 14602072

3. Feng, L. et al. (2004) The Journal of biological chemistry 279, 29681-90. PMID: 15051726

4. Yang, C. et al. (2006) Molecular cell 21, 135-41. PMID: 16387660

5. Chastagner, P. et al. (2006) EMBO reports 7, 1147-53. PMID: 17028573

6. Lee, TL. et al. (2008) Biochemical and biophysical research communications 375, 326-30. PMID: 18718448

7. Scialpi, F. et al. (2008) Biochemical pharmacology 76, 1515-21. PMID: 18718449

8. Bhandari, D. et al. (2009) Molecular biology of the cell 20, 1324-39. PMID: 19116316

9. Tao, M. et al. (2009) Current biology : CB 19, 1255-63. PMID: 19592251

10. You, F. et al. (2009) Nature immunology 10, 1300-8. PMID: 19881509

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human ITCH Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human ITCH Polyclonal Antibody (HP466014)

개요

이미지

참고 문헌

데이터시트

SDS