ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
17.64 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
E3 ubiquitin-protein ligase Itchy homolog is a ~102 kDa protein. Acts as an Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. Involved in the control of inflammatory signaling pathways. Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of the complex after TNF stimulation.
1. Winberg, G. et al. (2000) Molecular and cellular biology 20, 8526-35. PMID: 11046148
2. Marchese, A. et al. (2003) Developmental cell 5, 709-22. PMID: 14602072
3. Feng, L. et al. (2004) The Journal of biological chemistry 279, 29681-90. PMID: 15051726
4. Yang, C. et al. (2006) Molecular cell 21, 135-41. PMID: 16387660
5. Chastagner, P. et al. (2006) EMBO reports 7, 1147-53. PMID: 17028573
6. Lee, TL. et al. (2008) Biochemical and biophysical research communications 375, 326-30. PMID: 18718448
7. Scialpi, F. et al. (2008) Biochemical pharmacology 76, 1515-21. PMID: 18718449
8. Bhandari, D. et al. (2009) Molecular biology of the cell 20, 1324-39. PMID: 19116316
9. Tao, M. et al. (2009) Current biology : CB 19, 1255-63. PMID: 19592251
10. You, F. et al. (2009) Nature immunology 10, 1300-8. PMID: 19881509
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human ITCH Protein, N-His
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