Anti-Human ITCH Polyclonal Antibody (HP466014)

Name: Anti-Human ITCH Polyclonal Antibody
Brand: abinScience
SKU: HP466014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HP466014

Description

Anti-Human ITCH Polyclonal Antibody (HP466014) is a rabbit polyclonal antibody detecting ITCH in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ITCH (Met1-Gly141).

Target

Atrophin-1-interacting protein 4, HECT-type E3 ubiquitin transferase Itchy homolog, NFE2-associated polypeptide 1, NAPP1, E3 ubiquitin-protein ligase Itchy homolog, ITCH, Itch, AIP4

Purification

Purified by antigen affinity column.

Accession

Q96J02

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

E3 ubiquitin-protein ligase Itchy homolog is a ~102 kDa protein. Acts as an Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. Involved in the control of inflammatory signaling pathways. Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of the complex after TNF stimulation.

1. Winberg, G. et al. (2000) Molecular and cellular biology 20, 8526-35. PMID: 11046148

2. Marchese, A. et al. (2003) Developmental cell 5, 709-22. PMID: 14602072

3. Feng, L. et al. (2004) The Journal of biological chemistry 279, 29681-90. PMID: 15051726

4. Yang, C. et al. (2006) Molecular cell 21, 135-41. PMID: 16387660

5. Chastagner, P. et al. (2006) EMBO reports 7, 1147-53. PMID: 17028573

6. Lee, TL. et al. (2008) Biochemical and biophysical research communications 375, 326-30. PMID: 18718448

7. Scialpi, F. et al. (2008) Biochemical pharmacology 76, 1515-21. PMID: 18718449

8. Bhandari, D. et al. (2009) Molecular biology of the cell 20, 1324-39. PMID: 19116316

9. Tao, M. et al. (2009) Current biology : CB 19, 1255-63. PMID: 19592251

10. You, F. et al. (2009) Nature immunology 10, 1300-8. PMID: 19881509

Note

For research use only.

Images

References

Datasheet

Anti-Human ITCH Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-Human ITCH Polyclonal Antibody (HP466014)

Overview

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References

Datasheet

SDS

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