Anti-Human KPNB1 Polyclonal Antibody (HD617014)

Name: Anti-Human KPNB1 Polyclonal Antibody
Brand: abinScience
SKU: HD617014
Price: 168 USD
Availability: InStock

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100ug

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HD617014

설명

Anti-Human KPNB1 Polyclonal Antibody (HD617014) is a rabbit polyclonal antibody detecting KPNB1. Suitable for Mouse, Rat, and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human KPNB1 (Met1-Ala442).

타겟

Importin subunit beta-1, Importin-90, KPNB1, Karyopherin subunit beta-1, NTF97, Nuclear factor p97, PTAC97, Pore targeting complex 97 kDa subunit

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

Q14974

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

배경

Importin subunit beta-1 (KPNB1) is a ~97 kDa protein. Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

1. Jäkel, S. et al. (1999) The EMBO journal 18, 2411-23. PMID: 10228156

2. Dean, KA. et al. (2001) Journal of cell science 114, 3479-85. PMID: 11682607

3. White, WO. et al. (2002) Journal of cellular biochemistry 85, 42-53. PMID: 11891849

4. Mingot, JM. et al. (2009) Journal of cell science 122, 1452-60. PMID: 19386897

5. Pawłowski, R. et al. (2010) The EMBO journal 29, 3448-58. PMID: 20818336

6. Choi, S. et al. (2014) Acta crystallographica. Section D, Biological crystallography 70, 1050-60. PMID: 24699649

7. Chi, NC. et al. (1995) The Journal of cell biology 130, 265-74. PMID: 7615630

8. Jäkel, S. et al. (1998) The EMBO journal 17, 4491-502. PMID: 9687515

참고사항

For research use only

이미지

Western blot

Western blot analysis was performed using anti-KPNB1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: A2780s cell lysate
Lane 2: NIH3T3 cell lysate
Lane 3: Hela cell lysate
Lane 4: K562 cell lysate
Lane 5: A549 cell lysate
Lane 6: Caco2 cell lysate

참고 문헌

데이터시트

Anti-Human KPNB1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human KPNB1 Polyclonal Antibody (HD617014)

개요

이미지

참고 문헌

데이터시트

SDS