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Anti-Human MDK/Midkine Recombinant Antibody (SAA1776) (HB964013)

Anti-Human MDK/Midkine Recombinant Antibody (SAA1776)
Anti-Human MDK/Midkine Recombinant Antibody (SAA1776)
가격(USD):
사양:
  • 20ug
  • 50ug
  • 100ug
  • 1mg
수량:
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  • 개요

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  • 참고 문헌

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개요
카탈로그 번호HB964013
설명
Anti-Human MDK/Midkine Recombinant Antibody (SAA1776) (HB964013) is a mouse monoclonal antibody detecting MDK/Midkine in ELISA. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
종 반응성Human
응용 분야ELISA
숙주 종Mouse
클론성Monoclonal
이소타입IgG2a, kappa
클론 IDSAA1776
타겟 Amphiregulin-associated protein, Midgestation and kidney protein, NEGF2, MDK, ARAP, MK1, Neurite outgrowth-promoting factor 2, MK, Midkine, Neurite outgrowth-promoting protein
내독소 수준 Please contact with the lab for this information.
순도 >95% as determined by SDS-PAGE.
정제 Protein A/G purified from cell culture supernatant.
등록번호 P21741
RRID Anti-Human MDK/Midkine Antibody (SAA1776) (abinScience Cat# HB964013, RRID:AB_3727538)
형태 Liquid
저장 완충액 0.01M PBS pH 7.4

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:2000-1:20000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
배경

Midkine (MDK) is a ~15 kDa protein. Secreted protein that functions as a cytokine and growth factor and mediates its signal through cell-surface proteoglycan and non-proteoglycan receptors. Binds cell-surface proteoglycan receptors via their chondroitin sulfate (CS) groups. Thereby regulates many processes like inflammatory response, cell proliferation, cell adhesion, cell growth, cell survival, tissue regeneration, cell differentiation and cell migration. Participates in inflammatory processes by exerting two different activities. Firstly, mediates neutrophils and macrophages recruitment to the sites of inflammation both by direct action by cooperating namely with ITGB2 via LRP1 and by inducing chemokine expression.

1. Maeda, N. et al. (1999) The Journal of biological chemistry 274, 12474-9. PMID: 10212223
2. Muramatsu, H. et al. (2000) Biochemical and biophysical research communications 270, 936-41. PMID: 10772929
3. Kurosawa, N. et al. (2001) Glycoconjugate journal 18, 499-507. PMID: 12084985
4. Stoica, GE. et al. (2002) The Journal of biological chemistry 277, 35990-8. PMID: 12122009
5. Sakaguchi, N. et al. (2003) Neuroscience research 45, 219-24. PMID: 12573468
6. Muramatsu, H. et al. (2004) Journal of cell science 117, 5405-15. PMID: 15466886
7. Huang, Y. et al. (2008) Cell cycle (Georgetown, Tex.) 7, 1613-22. PMID: 18469519
8. Weckbach, LT. et al. (2014) Blood 123, 1887-96. PMID: 24458438
9. Horiba, M. et al. (2000) The Journal of clinical investigation 105, 489-95. PMID: 10683378
참고사항 For research use only.
Isotype Control Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173) (MF533507)
이미지
  • Anti-Human MDK/Midkine Recombinant Antibody (SAA1776)

    SDS-PAGE

    SDS-PAGE for Anti-Human MDK/Midkine Antibody (SAA1776)

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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