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| 카탈로그 번호 | HP278014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 종 반응성 | Human, Mouse | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human MEPCE (Lys407-His689). | ||||||||
| 타겟 | MEPCE, Bin3 homolog, Bicoid-interacting protein 3 homolog, 7SK snRNA methylphosphate capping enzyme, MePCE, BCDIN3 | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | Q7L2J0 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | 7SK snRNA methylphosphate capping enzyme (MEPCE) is a ~74 kDa protein. S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA (7SK RNA), leading to stabilize it. Also has a non-enzymatic function as part of the 7SK RNP complex: the 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC). In the 7SK RNP complex, MEPCE is required to stabilize 7SK RNA and facilitate the assembly of 7SK RNP complex. MEPCE has a non-enzymatic function in the 7SK RNP complex; interaction with LARP7 within the 7SK RNP complex occluding its catalytic center. 1. Jeronimo, C. et al. (2007) Molecular cell 27, 262-274. PMID: 17643375 2. Xue, Y. et al. (2010) Nucleic acids research 38, 360-9. PMID: 19906723 3. Yang, Y. et al. (2019) Nature chemical biology 15, 132-140. PMID: 30559425 4. Egloff, S. et al. (2017) The EMBO journal 36, 934-948. PMID: 28254838 5. Peng, Q. et al. (2023) Science advances 9, eadj9359. PMID: 38100593 | ||||||||
| 참고사항 | For research use only. |
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