Human MEPCE Recombinant Protein (N-His) (HP278012)

Name: Human MEPCE Recombinant Protein (N-His)
Brand: abinScience
SKU: HP278012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP278012
Description	Recombinant Human MEPCE Protein, N-His (HP278012) expressed in E. coli, spanning Lys407-His689. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q7L2J0
Protein length	Lys407-His689
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	34.68 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	MEPCE, Bin3 homolog, Bicoid-interacting protein 3 homolog, 7SK snRNA methylphosphate capping enzyme, MePCE, BCDIN3
Background	7SK snRNA methylphosphate capping enzyme (MEPCE) is a ~74 kDa protein. S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA (7SK RNA), leading to stabilize it. Also has a non-enzymatic function as part of the 7SK RNP complex: the 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC). In the 7SK RNP complex, MEPCE is required to stabilize 7SK RNA and facilitate the assembly of 7SK RNP complex. MEPCE has a non-enzymatic function in the 7SK RNP complex; interaction with LARP7 within the 7SK RNP complex occluding its catalytic center. 1. Jeronimo, C. et al. (2007) Molecular cell 27, 262-274. PMID: 17643375 2. Xue, Y. et al. (2010) Nucleic acids research 38, 360-9. PMID: 19906723 3. Yang, Y. et al. (2019) Nature chemical biology 15, 132-140. PMID: 30559425 4. Egloff, S. et al. (2017) The EMBO journal 36, 934-948. PMID: 28254838 5. Peng, Q. et al. (2023) Science advances 9, eadj9359. PMID: 38100593
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human MEPCE Protein, N-His

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HP278012-SDS-PAGE-1.jpg

Datasheet

Human MEPCE Recombinant Protein (N-His).pdf

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Human MEPCE Recombinant Protein (N-His) (HP278012)

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