Anti-Human MEPCE Polyclonal Antibody (HP278014)

Name: Anti-Human MEPCE Polyclonal Antibody
Brand: abinScience
SKU: HP278014
Price: 168 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HP278014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MEPCE (Lys407-His689).

Target

MEPCE, Bin3 homolog, Bicoid-interacting protein 3 homolog, 7SK snRNA methylphosphate capping enzyme, MePCE, BCDIN3

Purification

Purified by antigen affinity column.

Accession

Q7L2J0

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

7SK snRNA methylphosphate capping enzyme (MEPCE) is a ~74 kDa protein. S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA (7SK RNA), leading to stabilize it. Also has a non-enzymatic function as part of the 7SK RNP complex: the 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC). In the 7SK RNP complex, MEPCE is required to stabilize 7SK RNA and facilitate the assembly of 7SK RNP complex. MEPCE has a non-enzymatic function in the 7SK RNP complex; interaction with LARP7 within the 7SK RNP complex occluding its catalytic center.

1. Jeronimo, C. et al. (2007) Molecular cell 27, 262-274. PMID: 17643375

2. Xue, Y. et al. (2010) Nucleic acids research 38, 360-9. PMID: 19906723

3. Yang, Y. et al. (2019) Nature chemical biology 15, 132-140. PMID: 30559425

4. Egloff, S. et al. (2017) The EMBO journal 36, 934-948. PMID: 28254838

5. Peng, Q. et al. (2023) Science advances 9, eadj9359. PMID: 38100593

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human MEPCE polyclonal antibody at 1ug/mL on recombinant Human MEPCE (Catalog No. HP278012).

References

Datasheet

Anti-Human MEPCE Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human MEPCE Polyclonal Antibody (HP278014)

Overview

Images

References

Datasheet

SDS

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