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| 카탈로그 번호 | HW299013 | ||||||
|---|---|---|---|---|---|---|---|
| 종 반응성 | Human | ||||||
| 응용 분야 | Blocking, ELISA, ICC, IF, FCM, WB | ||||||
| 숙주 종 | Mouse | ||||||
| 클론성 | Monoclonal | ||||||
| 이소타입 | IgG1, kappa | ||||||
| 클론 ID | LEM-2/15 | ||||||
| 타겟 | MT-MMP 1, Matrix metalloproteinase-14, Membrane-type matrix metalloproteinase 1, Membrane-type-1 matrix metalloproteinase, MMP14, MTMMP1, MT1-MMP, MMP-14, MMP-X1, MT1MMP | ||||||
| 내독소 수준 | < 10 EU/mg | ||||||
| 순도 | >95% as determined by SDS-PAGE. | ||||||
| 정제 | Protein A/G purified from cell culture supernatant. | ||||||
| 등록번호 | P50281 | ||||||
| RRID | Anti-Human MMP14 Antibody (LEM-2/15) (abinScience Cat# HW299013, RRID:AB_3725683) |
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| 형태 | Liquid | ||||||
| 저장 완충액 | 0.01M PBS pH 7.4. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| 배경 | Matrix metalloproteinase-14 (MMP14) is a ~65 kDa protein. Endopeptidase that degrades various components of the extracellular matrix such as collagen. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development. Activates progelatinase A/MMP2, thereby acting as a positive regulator of cell growth and migration. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. May be involved in actin cytoskeleton reorganization by cleaving PTK7. 1. Sato, H. et al. (1994) Nature 370, 61-5. PMID: 8015608 2. Gu, G. et al. (2012) Journal of cellular biochemistry 113, 1013-21. PMID: 22065321 3. Noda, K. et al. (2003) Investigative ophthalmology & visual science 44, 2163-70. PMID: 12714657 4. Golubkov, VS. et al. (2010) The Journal of biological chemistry 285, 35740-9. PMID: 20837484 5. Jin, G. et al. (2011) The EMBO journal 30, 2281-93. PMID: 21572390 6. Cork, SM. et al. (2012) Oncogene 31, 5144-52. PMID: 22330140 7. Chow, CFW. et al. (2022) Nature metabolism 4, 203-212. PMID: 35177851 | ||||||
| 참고사항 | For research use only. | ||||||
| Isotype Control | Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207) |
Detects MMP14 in indirect ELISAs.
SDS-PAGE for Anti-Human MMP14 Antibody (LEM-2/15).
Flow-cytometry using anti-human MMP14 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MMP14 antibody monoclonal antibody (Catalog # HW299013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot analysis was performed using anti-MMP14 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human MMP14 (Catalog No: HW299022)
Lane 2: negative control
Flow-cytometry using anti-human MMP14 antibody. BXPC3 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human MMP14 monoclonal antibody (Catalog HW299013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
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