Anti-Human NELFE Polyclonal Antibody (HB121014)

Name: Anti-Human NELFE Polyclonal Antibody
Brand: abinScience
SKU: HB121014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HB121014

설명

Anti-Human NELFE Polyclonal Antibody (HB121014) is a rabbit polyclonal antibody detecting NELFE in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human NELFE (Gly261-Arg331).

타겟

RDBP, RD, NELF-E, RNA-binding protein RD, NELFE, Negative elongation factor E

정제

Purified by antigen affinity column.

등록번호

P18615

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Negative elongation factor E (NELFE) is a ~43 kDa protein. Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA.

1. Yamaguchi, Y. et al. (1999) Cell 97, 41-51. PMID: 10199401

2. Gibson, BA. et al. (2016) Science (New York, N.Y.) 353, 45-50. PMID: 27256882

3. Yamaguchi, Y. et al. (2002) Molecular and cellular biology 22, 2918-27. PMID: 11940650

4. Narita, T. et al. (2003) Molecular and cellular biology 23, 1863-73. PMID: 12612062

5. Rao, JN. et al. (2008) Biochemistry 47, 3756-61. PMID: 18303858

6. Vos, SM. et al. (2016) eLife 5. PMID: 27282391

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human NELFE Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human NELFE Polyclonal Antibody (HB121014)

개요

이미지

참고 문헌

데이터시트

SDS

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