Anti-Human NEU3/Sialidase-3 Polyclonal Antibody (HP187014)

Name: Anti-Human NEU3/Sialidase-3 Polyclonal Antibody
Brand: abinScience
SKU: HP187014
Price: 96 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HP187014

설명

Anti-Human NEU3/Sialidase-3 Polyclonal Antibody (HP187014) is a rabbit polyclonal antibody detecting Sialidase-3 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human NEU3/Sialidase-3 (Met1-Asn428).

타겟

N-acetyl-alpha-neuraminidase 3, NEU3, Sialidase-3, Ganglioside sialidasedis, Membrane sialidase

정제

Purified by antigen affinity column.

등록번호

Q9UQ49

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Sialidase-3 (NEU3) is a ~48 kDa protein. Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Displays high catalytic efficiency for gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3. Plays a role in the regulation of transmembrane signaling through the modulation of ganglioside content of the lipid bilayer and by direct interaction with signaling receptors, such as EGFR. Desialylates EGFR and activates downstream signaling in proliferating cells. Contributes to clathrin-mediated endocytosis by regulating sorting of endocytosed receptors to early and recycling endosomes.

1. Wada, T. et al. (1999) Biochemical and biophysical research communications 261, 21-7. PMID: 10405317

2. Monti, E. et al. (2000) The Biochemical journal 349, 343-51. PMID: 10861246

3. Wang, Y. et al. (2001) European journal of biochemistry 268, 2201-8. PMID: 11298736

4. Wang, Y. et al. (2002) The Journal of biological chemistry 277, 26252-9. PMID: 12011038

5. Yamaguchi, K. et al. (2005) The Biochemical journal 390, 85-93. PMID: 15847605

6. Albohy, A. et al. (2010) Glycobiology 20, 1127-38. PMID: 20511247

7. Rodriguez-Walker, M. et al. (2017) Scientific reports 7, 4167. PMID: 28646141

8. Wada, T. et al. (2007) Oncogene 26, 2483-90. PMID: 17334392

9. Mozzi, A. et al. (2015) Glycobiology 25, 855-68. PMID: 25922362

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human NEU3/Sialidase-3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human NEU3/Sialidase-3 Polyclonal Antibody (HP187014)

개요

이미지

참고 문헌

데이터시트

SDS