Anti-Human NSMCE2 Polyclonal Antibody (HA545014)

Name: Anti-Human NSMCE2 Polyclonal Antibody
Brand: abinScience
SKU: HA545014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HA545014

설명

Anti-Human NSMCE2 Polyclonal Antibody (HA545014) is a rabbit polyclonal antibody detecting NSMCE2 in WB, IHC, ELISA. Suitable for Human and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

WB, IHC, ELISA

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human NSMCE2 (Ser16-Glu247).

타겟

NSMCE2

내독소 수준

Please contact with the lab for this information.

순도

>90% as determined by SDS-PAGE.

정제

Purified by antigen affinity column.

등록번호

Q96MF7

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

대체 이름

E3 SUMO-protein ligase NSE2, EC:2.3.2.-, E3 SUMO-protein transferase NSE2, MMS21 homolog, hMMS21, Non-structural maintenance of chromosomes element 2 homolog, Non-SMC element 2 homolog, NSMCE2

배경

E3 SUMO-protein ligase NSE2 (NSMCE2) is a ~27 kDa protein. E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. Is not be required for the stability of the complex. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Acts as an E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TSNAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, RAD51AP1, and maybe the cohesin components RAD21 and STAG2.

1. Potts, PR. et al. (2005) Molecular and cellular biology 25, 7021-32. PMID: 16055714

2. Potts, PR. et al. (2006) The EMBO journal 25, 3377-88. PMID: 16810316

3. Potts, PR. et al. (2007) Nature structural & molecular biology 14, 581-90. PMID: 17589526

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-NSMCE2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate
Lane 2: A549 cell lysate
Lane 3: 293T cell lysate

참고 문헌

데이터시트

Anti-Human NSMCE2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human NSMCE2 Polyclonal Antibody (HA545014)

개요

이미지

참고 문헌

데이터시트

SDS