Human NSMCE2 Recombinant Protein (H-His) (HA545012)

Name: Human NSMCE2 Recombinant Protein (H-His)
Brand: abinScience
SKU: HA545012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA545012
Description	Recombinant Human NSMCE2 Protein, H-His (HA545012) expressed in E. coli, spanning Ser16-Glu247. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q96MF7
Protein length	Ser16-Glu247
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	28.76 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	E3 SUMO-protein ligase NSE2, EC:2.3.2.-, E3 SUMO-protein transferase NSE2, MMS21 homolog, hMMS21, Non-structural maintenance of chromosomes element 2 homolog, Non-SMC element 2 homolog, NSMCE2
Background	E3 SUMO-protein ligase NSE2 (NSMCE2) is a ~27 kDa protein. E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. Is not be required for the stability of the complex. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Acts as an E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TSNAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, RAD51AP1, and maybe the cohesin components RAD21 and STAG2. 1. Potts, PR. et al. (2005) Molecular and cellular biology 25, 7021-32. PMID: 16055714 2. Potts, PR. et al. (2006) The EMBO journal 25, 3377-88. PMID: 16810316 3. Potts, PR. et al. (2007) Nature structural & molecular biology 14, 581-90. PMID: 17589526
Note	For research use only.

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SDS-PAGE for Recombinant Human NSMCE2 Protein, H-His

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Datasheet

Human NSMCE2 Recombinant Protein (H-His).pdf

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Human NSMCE2 Recombinant Protein (H-His) (HA545012)

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