Anti-Human NSMCE2 Polyclonal Antibody (HA545014)

Name: Anti-Human NSMCE2 Polyclonal Antibody
Brand: abinScience
SKU: HA545014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HA545014

Description

Anti-Human NSMCE2 Polyclonal Antibody (HA545014) is a rabbit polyclonal antibody detecting NSMCE2 in WB, IHC, ELISA. Suitable for Human and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

WB, IHC, ELISA

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human NSMCE2 (Ser16-Glu247).

Target

NSMCE2

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Accession

Q96MF7

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

E3 SUMO-protein ligase NSE2, EC:2.3.2.-, E3 SUMO-protein transferase NSE2, MMS21 homolog, hMMS21, Non-structural maintenance of chromosomes element 2 homolog, Non-SMC element 2 homolog, NSMCE2

Background

E3 SUMO-protein ligase NSE2 (NSMCE2) is a ~27 kDa protein. E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. Is not be required for the stability of the complex. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Acts as an E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TSNAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, RAD51AP1, and maybe the cohesin components RAD21 and STAG2.

1. Potts, PR. et al. (2005) Molecular and cellular biology 25, 7021-32. PMID: 16055714

2. Potts, PR. et al. (2006) The EMBO journal 25, 3377-88. PMID: 16810316

3. Potts, PR. et al. (2007) Nature structural & molecular biology 14, 581-90. PMID: 17589526

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-NSMCE2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate
Lane 2: A549 cell lysate
Lane 3: 293T cell lysate

References

Datasheet

Anti-Human NSMCE2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human NSMCE2 Polyclonal Antibody (HA545014)

Overview

Images

References

Datasheet

SDS

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