Anti-Human ORF2p Polyclonal Antibody (HD130014)

Name: Anti-Human ORF2p Polyclonal Antibody
Brand: abinScience
SKU: HD130014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HD130014

설명

Anti-Human ORF2p Polyclonal Antibody (HD130014) is a rabbit polyclonal antibody detecting ORF2p. Suitable for Human.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human ORF2p (Met1-Lys240).

타겟

EC:2.7.7.49, EC:3.1.21.-, Endonuclease, LINE-1 retrotransposable element ORF2 protein, ORF2p, Reverse transcriptase

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

O00370

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

배경

LINE-1 retrotransposable element ORF2 protein (ORF2p) is a ~149 kDa protein. Has reverse transcriptase activity required for target-primed reverse transcription of the LINE-1 element mRNA, a crucial step in LINE-1 retrotransposition. Selectively binds and reversely transcribes RNA with a poly(A) tail consisting of at least 20 adenosines. Also has endonuclease activity that allows the introduction of nicks in the chromosomal target DNA. Cleaves DNA in AT-rich regions between a 5' stretch of purines and a 3' stretch of pyrimidines, corresponding to the sites of LINE-1 integration in the genome. Conformational properties of the target DNA sequence rather than specific nucleotides are key determinants of the ORF2p capacity for sequence-specific DNA recognition.

1. Thawani, A. et al. (2024) Nature 626, 186-193. PMID: 38096901

2. Baldwin, ET. et al. (2024) Nature 626, 194-206. PMID: 38096902

3. Dombroski, BA. et al. (1994) Molecular and cellular biology 14, 4485-92. PMID: 7516468

4. Sassaman, DM. et al. (1997) Nature genetics 16, 37-43. PMID: 9140393

5. Repanas, K. et al. (2007) Nucleic acids research 35, 4914-26. PMID: 17626046

6. Miller, I. et al. (2021) Nucleic acids research 49, 11350-11366. PMID: 34554261

7. Feng, Q. et al. (1996) Cell 87, 905-16. PMID: 8945517

참고사항

For research use only

이미지

참고 문헌

데이터시트

Anti-Human ORF2p Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human ORF2p Polyclonal Antibody (HD130014)

개요

이미지

참고 문헌

데이터시트

SDS