Anti-Human RAD1 Polyclonal Antibody (HT370014)

Name: Anti-Human RAD1 Polyclonal Antibody
Brand: abinScience
SKU: HT370014
Price: 168 USD
Availability: InStock

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개요
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개요

카탈로그 번호

HT370014

설명

Anti-Human RAD1 Polyclonal Antibody (HT370014) is a rabbit polyclonal antibody detecting RAD1 in ELISA, IHC, WB. Suitable for Human, Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human RAD1 (Met1-Ser282).

타겟

DNA repair exonuclease rad1 homolog, Rad1-like DNA damage checkpoint protein, RAD1, REC1, hRAD1, Cell cycle checkpoint protein RAD1

정제

Purified by antigen affinity column.

등록번호

O60671

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Cell cycle checkpoint protein RAD1 is a ~31 kDa protein. Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase.

1. Cai, RL. et al. (2000) The Journal of biological chemistry 275, 27909-16. PMID: 10846170

2. Rauen, M. et al. (2000) The Journal of biological chemistry 275, 29767-71. PMID: 10884395

3. Bermudez, VP. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 1633-8. PMID: 12578958

4. Smirnova, E. et al. (2005) The Biochemical journal 389, 13-7. PMID: 15871698

5. Toueille, M. et al. (2004) Nucleic acids research 32, 3316-24. PMID: 15314187

6. Wang, W. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 16762-7. PMID: 15556996

7. Cotta-Ramusino, C. et al. (2011) Science (New York, N.Y.) 332, 1313-7. PMID: 21659603

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human RAD1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human RAD1 Polyclonal Antibody (HT370014)

개요

이미지

참고 문헌

데이터시트

SDS