Anti-Human RHEB Polyclonal Antibody (HS914014)

Name: Anti-Human RHEB Polyclonal Antibody
Brand: abinScience
SKU: HS914014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HS914014

설명

Anti-Human RHEB Polyclonal Antibody (HS914014) is a rabbit polyclonal antibody detecting RHEB in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human RHEB (Met1-Met184).

타겟

RHEB2, Ras homolog enriched in brain, RHEB, GTP-binding protein Rheb

정제

Purified by antigen affinity column.

등록번호

Q15382

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

GTP-binding protein Rheb is a ~20 kDa protein. Small GTPase that acts as an allosteric activator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. In response to nutrients, growth factors or amino acids, specifically activates the protein kinase activity of MTOR, the catalytic component of the mTORC1 complex: acts by causing a conformational change that allows the alignment of residues in the active site of MTOR, thereby enhancing the phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1). RHEB is also required for localization of the TSC-TBC complex to lysosomal membranes. In response to starvation, RHEB is inactivated by the TSC-TBC complex, preventing activation of mTORC1. Has low intrinsic GTPase activity.

1. Inoki, K. et al. (2002) Nature cell biology 4, 648-57. PMID: 12172553

2. Tee, AR. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 13571-6. PMID: 12271141

3. Castro, AF. et al. (2003) The Journal of biological chemistry 278, 32493-6. PMID: 12842888

4. Inoki, K. et al. (2003) Genes & development 17, 1829-34. PMID: 12869586

5. Tee, AR. et al. (2003) Current biology : CB 13, 1259-68. PMID: 12906785

6. Li, Y. et al. (2004) Molecular and cellular biology 24, 7965-75. PMID: 15340059

7. Long, X. et al. (2005) Current biology : CB 15, 702-13. PMID: 15854902

8. Tee, AR. et al. (2005) FEBS letters 579, 4763-8. PMID: 16098514

9. Sancak, Y. et al. (2010) Cell 141, 290-303. PMID: 20381137

10. Mazhab-Jafari, MT. et al. (2012) Structure (London, England : 1993) 20, 1528-39. PMID: 22819219

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human RHEB Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human RHEB Polyclonal Antibody (HS914014)

개요

이미지

참고 문헌

데이터시트

SDS

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