Anti-Human RHOA Polyclonal Antibody (HX184014)

Name: Anti-Human RHOA Polyclonal Antibody
Brand: abinScience
SKU: HX184014
Price: 168 USD
Availability: InStock

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개요
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개요

카탈로그 번호

HX184014

설명

Anti-Human RHOA Polyclonal Antibody (HX184014) is a rabbit polyclonal antibody detecting RHOA in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human RHOA (Ala2-Gly189).

타겟

Rho cDNA clone 12, ARH12, RHO12, ARHA, RHOA, Transforming protein RhoA, h12

정제

Purified by antigen affinity column.

등록번호

P61586

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Transforming protein RhoA is a ~21 kDa protein. Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Mainly associated with cytoskeleton organization, in active state binds to a variety of effector proteins to regulate cellular responses such as cytoskeletal dynamics, cell migration and cell cycle. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation.

1. Wei, J. et al. (2013) Biochimica et biophysica acta 1833, 2757-2764. PMID: 23871831

2. Vabres, P. et al. (2019) Nature genetics 51, 1438-1441. PMID: 31570889

3. Quilliam, LA. et al. (1996) The Journal of biological chemistry 271, 28772-6. PMID: 8910519

4. Vincent, S. et al. (1997) Molecular and cellular biology 17, 2247-56. PMID: 9121475

5. Wing, MR. et al. (2003) The Journal of biological chemistry 278, 41253-8. PMID: 12900402

6. Kamijo, K. et al. (2006) Molecular biology of the cell 17, 43-55. PMID: 16236794

7. Wallace, SW. et al. (2011) Molecular and cellular biology 31, 81-91. PMID: 20974804

8. Dubash, AD. et al. (2013) The Journal of cell biology 202, 653-66. PMID: 23940119

9. Bristow, JM. et al. (2009) Journal of cell science 122, 4535-46. PMID: 19934221

10. Zaoui, K. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 18517-22. PMID: 20937854

참고사항

For research use only.

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참고 문헌

데이터시트

Anti-Human RHOA Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human RHOA Polyclonal Antibody (HX184014)

개요

이미지

참고 문헌

데이터시트

SDS

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