Anti-Human SGMS1 Polyclonal Antibody (HP580014)

Name: Anti-Human SGMS1 Polyclonal Antibody
Brand: abinScience
SKU: HP580014
Price: 168 USD
Availability: InStock

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개요
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개요

카탈로그 번호

HP580014

설명

Anti-Human SGMS1 Polyclonal Antibody (HP580014) is a rabbit polyclonal antibody detecting SGMS1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human SGMS1 (Lys2-Ala81).

타겟

Sphingomyelin synthase 1, Medulla oblongata-derived protein, Protein Mob, Transmembrane protein 23, Phosphatidylcholine:ceramide cholinephosphotransferase 1, TMEM23, MOB, SMS1, SGMS1

정제

Purified by antigen affinity column.

등록번호

Q86VZ5

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Phosphatidylcholine:ceramide cholinephosphotransferase 1 (SGMS1) is a ~48 kDa protein. Major sphingomyelin synthase at the Golgi apparatus. Catalyzes the reversible transfer of phosphocholine moiety in sphingomyelin biosynthesis: in the forward reaction transfers phosphocholine head group of phosphatidylcholine (PC) on to ceramide (CER) to form ceramide phosphocholine (sphingomyelin, SM) and diacylglycerol (DAG) as by-product, and in the reverse reaction transfers phosphocholine from SM to DAG to form PC and CER. The direction of the reaction depends on the levels of CER and DAG in Golgi membranes. Converts the newly synthesized CER, that is transported from the endoplasmic reticulum to the trans-Golgi by the Cer transport protein (CERT), to SM. Can form a heteromeric complex with glucosylceramide synthase (GCS) increasing SMS activity and reducing glucosylceramide synthesis, a critical mechanism that controls the metabolic fate of CER in the Golgi.

1. Huitema, K. et al. (2004) The EMBO journal 23, 33-44. PMID: 14685263

2. Tafesse, FG. et al. (2007) The Journal of biological chemistry 282, 17537-47. PMID: 17449912

3. Yamaoka, S. et al. (2004) The Journal of biological chemistry 279, 18688-93. PMID: 14976195

4. Ding, T. et al. (2008) Journal of lipid research 49, 376-85. PMID: 17982138

5. Ternes, P. et al. (2009) Journal of lipid research 50, 2270-7. PMID: 19454763

6. Hayashi, Y. et al. (2018) The Journal of biological chemistry 293, 17505-17522. PMID: 30242129

참고사항

For research use only.

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참고 문헌

데이터시트

Anti-Human SGMS1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human SGMS1 Polyclonal Antibody (HP580014)

개요

이미지

참고 문헌

데이터시트

SDS