Anti-Human SPOP Polyclonal Antibody (HT175014)

Name: Anti-Human SPOP Polyclonal Antibody
Brand: abinScience
SKU: HT175014
Price: 168 USD
Availability: InStock

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개요
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개요

카탈로그 번호

HT175014

설명

Anti-Human SPOP Polyclonal Antibody (HT175014) is a rabbit polyclonal antibody detecting SPOP in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Danio rerio, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human SPOP (Ser21-Thr175).

타겟

Roadkill homolog 1, HIB homolog 1, SPOP, Speckle-type POZ protein

정제

Purified by antigen affinity column.

등록번호

O43791

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Speckle-type POZ protein (SPOP) is a ~42 kDa protein. Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of MACROH2A1 and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL.

1. Nabais Sá, MJ. et al. (2020) American journal of human genetics 106, 405-411. PMID: 32109420

2. Sanada, S. et al. (2023) Biochemical and biophysical research communications 651, 30-38. PMID: 36791496

3. Schwartz, I. et al. (2023) eLife 12. PMID: 37622993

4. Zang, L. et al. (2023) Journal of virology 97, e0078623. PMID: 37796126

5. Pi, Y. et al. (2023) Journal of medical virology 95, e29254. PMID: 38018242

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human SPOP Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human SPOP Polyclonal Antibody (HT175014)

개요

이미지

참고 문헌

데이터시트

SDS

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