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| 카탈로그 번호 | HX013013 | ||||
|---|---|---|---|---|---|
| 종 반응성 | Human | ||||
| 응용 분야 | ELISA, FCM, IF, IP | ||||
| 숙주 종 | Mouse | ||||
| 클론성 | Monoclonal | ||||
| 이소타입 | IgG1 | ||||
| 클론 ID | SAA0239 | ||||
| 타겟 | WDR5, BMP2-induced 3-kb gene protein, WD repeat-containing protein 5, BIG3 | ||||
| 내독소 수준 | Please contact with the lab for this information. | ||||
| 순도 | >95% as determined by SDS-PAGE. | ||||
| 정제 | Protein A/G purified from cell culture supernatant. | ||||
| 등록번호 | P61964 | ||||
| RRID | Anti-Human WDR5 IP Antibody (SAA0239) (abinScience Cat# HX013013, RRID:AB_3729773) |
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| 형태 | Liquid | ||||
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 배경 | WD repeat-containing protein 5 (WDR5) is a ~36 kDa protein. Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. 1. Han, Z. et al. (2006) Molecular cell 22, 137-44. PMID: 16600877 2. Couture, JF. et al. (2006) Nature structural & molecular biology 13, 698-703. PMID: 16829960 3. Guelman, S. et al. (2009) Molecular and cellular biology 29, 1176-88. PMID: 19103755 4. Garapaty, S. et al. (2009) The Journal of biological chemistry 284, 7542-52. PMID: 19131338 5. Patel, A. et al. (2009) The Journal of biological chemistry 284, 24242-56. PMID: 19556245 6. Cai, Y. et al. (2010) The Journal of biological chemistry 285, 4268-72. PMID: 20018852 7. Song, JJ. et al. (2008) The Journal of biological chemistry 283, 35258-64. PMID: 18840606 8. Avdic, V. et al. (2011) Structure (London, England : 1993) 19, 101-8. PMID: 21220120 9. Zhang, P. et al. (2012) Nucleic acids research 40, 4237-46. PMID: 22266653 | ||||
| 참고사항 | For research use only. | ||||
| Isotype Control | Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207) |
Flow-cytometry using anti-human WDR5 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human WDR5 antibody monoclonal antibody (Catalog # HX013013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human WDR5 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human WDR5 antibody monoclonal antibody (Catalog # HX013013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for Anti-Human WDR5 IP Antibody (SAA0239).
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