Anti-MCM5 Polyclonal Antibody (HW592014)

Name: Anti-MCM5 Polyclonal Antibody
Brand: abinScience
SKU: HW592014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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개요

카탈로그 번호

HW592014

설명

Anti-MCM5 Polyclonal Antibody (HW592014) is a rabbit polyclonal antibody detecting MCM5 in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, Xenopus tropicalis, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human MCM5 (Val331-Ile537).

타겟

CDC46, DNA replication licensing factor MCM5, CDC46 homolog, P1-CDC46, MCM5

정제

Purified by antigen affinity column.

등록번호

P33992

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

DNA replication licensing factor MCM5 is a ~82 kDa protein. Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

1. Li, Z. et al. (2025) The EMBO journal 44, 6019-6050. PMID: 40940420

2. Tsuji, T. et al. (2006) Molecular biology of the cell 17, 4459-72. PMID: 16899510

3. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425

4. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004

5. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328

6. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-MCM5 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-MCM5 Polyclonal Antibody (HW592014)

개요

이미지

참고 문헌

데이터시트

SDS

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