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| 카탈로그 번호 | MP369107 |
|---|---|
| 종 반응성 | Mouse |
| 응용 분야 | FCM, FuncS, IF, IP |
| 숙주 종 | Rat |
| 클론성 | Monoclonal |
| 이소타입 | IgG2b, kappa |
| 클론 ID | 2.4G2 |
| 타겟 | Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III, Fc-gamma RIII, FcRIII, CD16, Low affinity immunoglobulin gamma Fc region receptor II, Fc gamma receptor IIB, Fc-gamma RII, Fc-gamma-RIIB, FcRII, IgG Fc receptor II beta, Lymphocyte antigen 17, Ly-17, CD32, Fcgr2, Fcgr2b, Ly-17 |
| 내독소 수준 | <1 EU/mg |
| 순도 | >95% as determined by SDS-PAGE. |
| 관련 제품 | pH 7.4 Dilution Buffer |
| 정제 | Protein A/G purified from cell culture supernatant. |
| 등록번호 | P08508, P08101 |
| 형태 | Liquid |
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| 배경 | Low affinity immunoglobulin gamma Fc region receptor III (CD16/CD32) is a ~30 kDa protein. Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor which binds to IgG1, IgG2a and IgG2b. Mediates neutrophil activation by IgG complexes redundantly with Fcgr4. 1. Hirano, M. et al. (2007) Nature immunology 8, 762-71. PMID: 17558411 2. Jakus, Z. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 180, 618-29. PMID: 18097064 |
| 참고사항 | For research use only. |
Detects CD16/CD32 in indirect ELISAs.
Flow-cytometry using anti-mouse CD16 antibody.CD16 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD16 antibody monoclonal antibody (Catalog # MP369107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rat antibody and cells analysed on a NovoCyte Flow Cytometer.
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