Anti-Mouse LAG-3 Recombinant Nanobody (Clone:3132) (MB613023)

Name: Anti-Mouse LAG-3 Recombinant Nanobody (Clone:3132)
Brand: abinScience
SKU: MB613023
Price: 126 USD
Availability: InStock

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20ug
50ug
100ug
1mg

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

MB613023

설명

Anti-Mouse CD223/LAG3 Nanobody (Clone:3132) [Clone:3132] (MB613023) is a alpaca monoclonal antibody detecting CD223 in ELISA, FuncS. Suitable for Mouse.

종 반응성

Mouse

응용 분야

ELISA, FuncS

숙주 종

Alpaca

클론성

Monoclonal

이소타입

VHH-8His-Cys-tag

클론 ID

Clone:3132

타겟

CD223, FDC, LAG-3, LAG3, Lymphocyte activation gene 3 protein, Secreted lymphocyte activation gene 3 protein, sLAG-3

내독소 수준

< 10 EU/mg

순도

>95% as determined by SDS-PAGE.

정제

Purified by Nickel column.

등록번호

Q61790

형태

Liquid

저장 완충액

0.01M PBS pH 7.4.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:2000-1:20000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Lymphocyte activation gene 3 protein (CD223/LAG3) is a ~56 kDa protein. Inhibitory receptor on antigen activated T-cells. Delivers inhibitory signals upon binding to ligands, such as MHC class II, its main ligand present at the surface of antigen-presenting cells (APCs), and FGL1, which is secreted by hepatocytes and certain types of tumor cells. Ligand-binding initiates a signaling that inhibits the T-cell receptor (TCR) in the immunological synapse, preventing T-cell activation. Mechanistically, ligand-binding promotes (1) ubiquitination of the KIEELE motif, unleashing the RRFSALE motif from the membrane and (2) leading to the formation of condensates with the TCR component CD3E, thereby disrupting the association between CD3E and LCK and preventing TCR activation. May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1. CD223 is the therapeutic target of relatlimab (Opdualag).

1. Workman, CJ. et al. (2002) European journal of immunology 32, 2255-63. PMID: 12209638

2. Workman, CJ. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 169, 5392-5. PMID: 12421911

3. Workman, CJ. et al. (2003) European journal of immunology 33, 970-9. PMID: 12672063

4. Workman, CJ. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 172, 5450-5. PMID: 15100286

5. Workman, CJ. et al. (2005) Journal of immunology (Baltimore, Md. : 1950) 174, 688-95. PMID: 15634887

6. Wang, J. et al. (2019) Cell 176, 334-347.e12. PMID: 30580966

7. Maruhashi, T. et al. (2022) Immunity 55, 912-924.e8. PMID: 35413245

8. Ming, Q. et al. (2022) Nature immunology 23, 1031-1041. PMID: 35761082

9. Silberstein, JL. et al. (2024) Proceedings of the National Academy of Sciences of the United States of America 121, e2310866121. PMID: 38483996

10. Ming, Q. et al. (2024) Nature communications 15, 7513. PMID: 39209860

참고사항

For research use only.

이미지

SDS-PAGE

SDS-PAGE for Anti-Mouse CD223/LAG3 Nanobody (Clone:3132)

참고 문헌

데이터시트

Anti-Mouse LAG-3 Recombinant Nanobody (Clone:3132).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Mouse LAG-3 Recombinant Nanobody (Clone:3132) (MB613023)

개요

이미지

참고 문헌

데이터시트

SDS

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