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| 카탈로그 번호 | MB945013 | ||||
|---|---|---|---|---|---|
| 종 반응성 | Human, Mouse | ||||
| 응용 분야 | ELISA, IP | ||||
| 숙주 종 | Mouse | ||||
| 클론성 | Monoclonal | ||||
| 이소타입 | IgG2a, kappa | ||||
| 클론 ID | SDS3 | ||||
| 테스트된 응용 분야 | FCM | ||||
| 타겟 | Matrix metalloproteinase-9, CLG4B, GELB, 92 kDa type IV collagenase, 92 kDa gelatinase, Gelatinase B, MMP9, MMP-9 | ||||
| 내독소 수준 | Please contact with the lab for this information. | ||||
| 순도 | >95% as determined by SDS-PAGE. | ||||
| 정제 | Protein A/G purified from cell culture supernatant. | ||||
| 등록번호 | P41245 | ||||
| RRID | Anti-Mouse MMP9 Antibody (SDS3) (abinScience Cat# MB945013, RRID:AB_3725633) |
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| 형태 | Liquid | ||||
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 배경 | Matrix metalloproteinase-9 (MMP9) is a ~80 kDa protein. Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. 1. Reponen, P. et al. (1994) The Journal of cell biology 124, 1091-1102. PMID: 8132709 2. Ahn, BJ. et al. (2012) Biochemical and biophysical research communications 428, 438-44. PMID: 23142597 3. Jeon, S. et al. (2020) Circulation 142, 1736-1751. PMID: 32883094 | ||||
| 참고사항 | For research use only. | ||||
| Isotype Control | Mouse IgG2a Recombinant Isotype Control Antibody (S43.10) (MF533307) |
SDS-PAGE for Anti-Mouse MMP9 Antibody (SDS3).
Flow-cytometry using PE anti-human MMP2 antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human MMP2 monoclonal antibody (Catalog: MB945013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using PE anti-human MMP9 antibody. BxPC-3 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human MMP9 monoclonal antibody (Catalog: MB945013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
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