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| 카탈로그 번호 | HV187014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-NFKBID Polyclonal Antibody (HV187014) is a rabbit polyclonal antibody detecting NFKBID in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
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| 종 반응성 | Human | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human NFKBID (Asp46-Val285). | ||||||||
| 타겟 | NFKB inhibitor delta, IkB-delta, TA-NFKBH, NF-kappa-B inhibitor delta, IkappaBNS, I-kappa-B-delta, T-cell activation NFKB-like protein, IKBNS, IkappaBdelta, NFKBID | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | Q8NI38 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | NF-kappa-B inhibitor delta (NFKBID) is a ~33 kDa protein. Regulates the expression of IL-2, IL-6, and other cytokines through regulation on NF-kappa-B activity. Functions in the regulation of inflammatory responses. Involved in the induction of T helper 17 cells (Th17) differentiation upon recognition of antigen by T cell antigen receptor (TCR). May also regulate TCR-induced negative selection of thymocytes. | ||||||||
| 참고사항 | For research use only. |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting NFKBID (HV187014) at a concentration of 12.6 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of NFKBID was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
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