Anti-NR5A2 Polyclonal Antibody (HT142014)

Name: Anti-NR5A2 Polyclonal Antibody
Brand: abinScience
SKU: HT142014
Price: 168 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD): --

사양:

50ug
100ug

수량:

문의하기

개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HT142014

설명

Anti-NR5A2 Polyclonal Antibody (HT142014) is a rabbit polyclonal antibody detecting NR5A2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human NR5A2 (Ser300-Ala541).

타겟

Liver receptor homolog 1, hB1F, NR5A2, Alpha-1-fetoprotein transcription factor, CYP7A promoter-binding factor, B1-binding factor, FTF, Nuclear receptor subfamily 5 group A member 2, LRH-1, B1F, CPF, Hepatocytic transcription factor

정제

Purified by antigen affinity column.

등록번호

O00482

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Nuclear receptor subfamily 5 group A member 2 (NR5A2) is a ~61 kDa protein. Orphan nuclear receptor that binds DNA as a monomer to the 5'-TCAAGGCCA-3' sequence and controls expression of target genes: regulates key biological processes, such as early embryonic development, cholesterol and bile acid synthesis pathways, as well as liver and pancreas morphogenesis. Ligand-binding causes conformational change which causes recruitment of coactivators, promoting target gene activation. The specific ligand is unknown, but specific phospholipids, such as phosphatidylethanolamine, phosphatidylserine, dilauroyl phosphatidylcholine and diundecanoyl phosphatidylcholine can act as ligand in vitro. Acts as a pioneer transcription factor, which unwraps target DNA from histones and elicits local opening of closed chromatin. Plays a central role during preimplantation stages of embryonic development.

1. Solomon, IH. et al. (2005) Journal of molecular biology 354, 1091-102. PMID: 16289203

2. Burendahl, S. et al. (2008) Biochemistry 47, 5205-15. PMID: 18410128

3. Lee, JM. et al. (2011) Nature 474, 506-10. PMID: 21614002

4. Seacrist, CD. et al. (2020) Structure (London, England : 1993) 28, 830-846.e9. PMID: 32433991

5. Kobayashi, W. et al. (2024) Nature structural & molecular biology 31, 757-766. PMID: 38409506

6. Li, M. et al. (1998) The Journal of biological chemistry 273, 29022-31. PMID: 9786908

7. Krylova, IN. et al. (2005) Cell 120, 343-55. PMID: 15707893

8. Ortlund, EA. et al. (2005) Nature structural & molecular biology 12, 357-63. PMID: 15723037

9. Wang, W. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 7505-10. PMID: 15897460

10. Musille, PM. et al. (2012) Nature structural & molecular biology 19, 532-S2. PMID: 22504882

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-NR5A2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-NR5A2 Polyclonal Antibody (HT142014)

개요

이미지

참고 문헌

데이터시트

SDS