ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
Liver receptor homolog 1, hB1F, NR5A2, Alpha-1-fetoprotein transcription factor, CYP7A promoter-binding factor, B1-binding factor, FTF, Nuclear receptor subfamily 5 group A member 2, LRH-1, B1F, CPF, Hepatocytic transcription factor
Background
Nuclear receptor subfamily 5 group A member 2 (NR5A2) is a ~61 kDa protein. Orphan nuclear receptor that binds DNA as a monomer to the 5'-TCAAGGCCA-3' sequence and controls expression of target genes: regulates key biological processes, such as early embryonic development, cholesterol and bile acid synthesis pathways, as well as liver and pancreas morphogenesis. Ligand-binding causes conformational change which causes recruitment of coactivators, promoting target gene activation. The specific ligand is unknown, but specific phospholipids, such as phosphatidylethanolamine, phosphatidylserine, dilauroyl phosphatidylcholine and diundecanoyl phosphatidylcholine can act as ligand in vitro. Acts as a pioneer transcription factor, which unwraps target DNA from histones and elicits local opening of closed chromatin. Plays a central role during preimplantation stages of embryonic development.
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2. Burendahl, S. et al. (2008) Biochemistry 47, 5205-15. PMID: 18410128
3. Lee, JM. et al. (2011) Nature 474, 506-10. PMID: 21614002
4. Seacrist, CD. et al. (2020) Structure (London, England : 1993) 28, 830-846.e9. PMID: 32433991
5. Kobayashi, W. et al. (2024) Nature structural & molecular biology 31, 757-766. PMID: 38409506
6. Li, M. et al. (1998) The Journal of biological chemistry 273, 29022-31. PMID: 9786908
7. Krylova, IN. et al. (2005) Cell 120, 343-55. PMID: 15707893
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