Anti-PKM Polyclonal Antibody (HB819014)

Name: Anti-PKM Polyclonal Antibody
Brand: abinScience
SKU: HB819014
Price: 168 USD
Availability: InStock

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개요
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개요

카탈로그 번호

HB819014

설명

Anti-PKM Polyclonal Antibody (HB819014) is a rabbit polyclonal antibody detecting PKM in ELISA, IHC, WB. Suitable for Human, Mouse, Cat, Rat, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human PKM (Met1-Pro531).

타겟

PK2, Opa-interacting protein 3, THBP1, Tumor M2-PK, PKM, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, PK3, Pyruvate kinase 2/3, p58, CTHBP, PKM2, Pyruvate kinase PKM, OIP-3, Cytosolic thyroid hormone-binding protein, OIP3

정제

Purified by antigen affinity column.

등록번호

P14618

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Pyruvate kinase PKM is a ~57 kDa protein. Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

1. Dombrauckas, JD. et al. (2005) Biochemistry 44, 9417-29. PMID: 15996096

2. Ashizawa, K. et al. (1991) Biochemistry 30, 7105-11. PMID: 1854723

3. Vander Heiden, MG. et al. (2010) Science (New York, N.Y.) 329, 1492-9. PMID: 20847263

참고사항

For research use only.

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참고 문헌

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Anti-PKM Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-PKM Polyclonal Antibody (HB819014)

개요

이미지

참고 문헌

데이터시트

SDS

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