Anti-Human PKM Recombinant Antibody (SAA1958) (HB819013)

Name: Anti-Human PKM Recombinant Antibody (SAA1958)
Brand: abinScience
SKU: HB819013
Price: 196 USD
Availability: InStock

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100ug
1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB819013

Species reactivity

Human

Applications

ELISA

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SAA1958

Clonality

Monoclonal

Target

PK2, Opa-interacting protein 3, THBP1, Tumor M2-PK, PKM, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, PK3, Pyruvate kinase 2/3, p58, CTHBP, PKM2, Pyruvate kinase PKM, OIP-3, Cytosolic thyroid hormone-binding protein, OIP3

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P14618

RRID

Anti-Human PKM Antibody (SAA1958) (abinScience Cat# HB819013, RRID:AB_3727722)
_x005F_x000D_

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Pyruvate kinase PKM is a ~57 kDa protein. Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

1. Dombrauckas, JD. et al. (2005) Biochemistry 44, 9417-29. PMID: 15996096

2. Ashizawa, K. et al. (1991) Biochemistry 30, 7105-11. PMID: 1854723

3. Vander Heiden, MG. et al. (2010) Science (New York, N.Y.) 329, 1492-9. PMID: 20847263

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human PKM Antibody (SAA1958)

References

Datasheet

Anti-Human PKM Recombinant Antibody (SAA1958).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human PKM Recombinant Antibody (SAA1958) (HB819013)

Overview

Images

References

Datasheet

SDS

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