Anti-SARS-CoV-2 ORF3a Polyclonal Antibody (VK577014)

Name: Anti-SARS-CoV-2 ORF3a Polyclonal Antibody
Brand: abinScience
SKU: VK577014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

VK577014

설명

Anti-SARS-CoV-2 ORF3a Polyclonal Antibody (VK577014) is a rabbit polyclonal antibody detecting ORF3a protein in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant SARS-CoV-2 ORF3a (Leu115-Leu275).

타겟

ORF3a protein, ORF3a, Accessory protein 3a, Protein 3a, Protein U274, Protein X1, 3a

정제

Purified by antigen affinity column.

등록번호

P0DTC3

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

ORF3a protein is a ~31 kDa protein. Plays a role in viral egress via lysosomal trafficking. Forms homotetrameric ion channels (viroporins) localized at endosomes and lysosomes, that may induce deacidification of lysosomes, allowing safe egress of virions via lysosomal trafficking. Also blocks autolysosome formation by binding and sequestering the host component VPS39 for homotypic fusion and protein sorting (HOPS) on late endosomes. This prevents fusion of autophagosomes with lysosomes, disrupting autophagy and facilitating virus egress. Induces host RETREG1/FAM134B-dependent reticulophagy by interacting with host HMGB1 and enhancing the association between HMGB1 and host BECN1.

1. Ghosh, S. et al. (2020) Cell 183, 1520-1535.e14. PMID: 33157038

2. Miao, G. et al. (2021) Developmental cell 56, 427-442.e5. PMID: 33422265

3. Kern, DM. et al. (2021) Nature structural & molecular biology 28, 573-582. PMID: 34158638

4. Zhang, X. et al. (2022) Autophagy 18, 2576-2592. PMID: 35239449

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-SARS-CoV-2 ORF3a Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-SARS-CoV-2 ORF3a Polyclonal Antibody (VK577014)

개요

이미지

참고 문헌

데이터시트

SDS

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