SARS-CoV-2 ORF3a Recombinant Protein (N-His) (VK577012)

Name: SARS-CoV-2 ORF3a Recombinant Protein (N-His)
Brand: abinScience
SKU: VK577012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	VK577012
Description	Recombinant SARS-CoV-2 ORF3a Protein, N-His (VK577012) expressed in E. coli, spanning Leu115-Leu275. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P0DTC3
Protein length	Leu115-Leu275
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	20.79 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ORF3a protein, ORF3a, Accessory protein 3a, Protein 3a, Protein U274, Protein X1, 3a
Background	ORF3a protein is a ~31 kDa protein. Plays a role in viral egress via lysosomal trafficking. Forms homotetrameric ion channels (viroporins) localized at endosomes and lysosomes, that may induce deacidification of lysosomes, allowing safe egress of virions via lysosomal trafficking. Also blocks autolysosome formation by binding and sequestering the host component VPS39 for homotypic fusion and protein sorting (HOPS) on late endosomes. This prevents fusion of autophagosomes with lysosomes, disrupting autophagy and facilitating virus egress. Induces host RETREG1/FAM134B-dependent reticulophagy by interacting with host HMGB1 and enhancing the association between HMGB1 and host BECN1. 1. Ghosh, S. et al. (2020) Cell 183, 1520-1535.e14. PMID: 33157038 2. Miao, G. et al. (2021) Developmental cell 56, 427-442.e5. PMID: 33422265 3. Kern, DM. et al. (2021) Nature structural & molecular biology 28, 573-582. PMID: 34158638 4. Zhang, X. et al. (2022) Autophagy 18, 2576-2592. PMID: 35239449
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant SARS-CoV-2 ORF3a Protein, N-His

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VK577012-SDS-PAGE-1.jpg

Datasheet

SARS-CoV-2 ORF3a Recombinant Protein (N-His).pdf

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SARS-CoV-2 ORF3a Recombinant Protein (N-His) (VK577012)

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