Anti-Streptococcus pyogenes Cas9 Polyclonal Antibody (JN045014)

Name: Anti-Streptococcus pyogenes Cas9 Polyclonal Antibody
Brand: abinScience
SKU: JN045014
Price: 168 USD
Availability: InStock

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개요

카탈로그 번호

JN045014

설명

Anti-Streptococcus pyogenes Cas9 Polyclonal Antibody (JN045014) is a rabbit polyclonal antibody detecting cas9 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Streptococcus pyogenes

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Streptococcus pyogenes Cas9 (Met1-Asp1368).

타겟

CRISPR-associated endonuclease Cas9/Csn1, 3.1.-.-, SpCas9, SpyCas9, cas9, csn1, SPy_1046

정제

Purified by antigen affinity column.

등록번호

Q99ZW2

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

CRISPR-associated endonuclease Cas9/Csn1 is a ~158 kDa protein. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA; Cas9 only stabilizes the pre-crRNA:tracrRNA interaction and has no catalytic function in RNA processing.

1. Deltcheva, E. et al. (2011) Nature 471, 602-7. PMID: 21455174

2. Fonfara, I. et al. (2014) Nucleic acids research 42, 2577-90. PMID: 24270795

3. Sternberg, SH. et al. (2014) Nature 507, 62-7. PMID: 24476820

참고사항

For research use only.

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