Anti-SV40 Large T Antigen Polyclonal Antibody (VK099024)

Name: Anti-SV40 Large T Antigen Polyclonal Antibody
Brand: abinScience
SKU: VK099024
Price: 168 USD
Availability: InStock

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사양:

50ug
100ug

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

VK099024

설명

Anti-SV40 Large T Antigen Polyclonal Antibody (VK099024) is a rabbit polyclonal antibody detecting Large T antigen in ELISA, IHC, WB. Suitable for Simian virus 40.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Simian virus 40 (SV40)

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant SV40 Large T Antigen (Gly251-Asp627).

타겟

Large T antigen, LT, LT-AG, 5.6.2.4, DNA 3'-5' helicase large T antigen

내독소 수준

Please contact with the lab for this information.

순도

>95% as determined by SDS-PAGE.

정제

Purified by antigen affinity column.

등록번호

P03070

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Large T antigen is a ~81 kDa protein. Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53.

1. Goetz, GS. et al. (1988) The Journal of biological chemistry 263, 383-92. PMID: 2826443

2. Wiekowski, M. et al. (1988) The Journal of biological chemistry 263, 436-42. PMID: 2826446

참고사항

For research use only

이미지

Western blot

Lane 1: recombinant SV40 Large T Antigen (Catalog No: VK099022)

참고 문헌

데이터시트

Anti-SV40 Large T Antigen Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-SV40 Large T Antigen Polyclonal Antibody (VK099024)

개요

이미지

참고 문헌

데이터시트

SDS