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Anti-WNT3A Polyclonal Antibody (HX182014)

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개요
카탈로그 번호HX182014
설명
Anti-WNT3A Polyclonal Antibody (HX182014) is a rabbit polyclonal antibody detecting WNT3A in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Chicken, and Meleagris gallopavo.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
종 반응성Human, Mouse
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human WNT3A (Ser19-Lys352).
타겟 Protein Wnt-3a, WNT3A
내독소 수준 Please contact with the lab for this information.
정제 Purified by antigen affinity column.
등록번호 P56704
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Protein Wnt-3a (WNT3A) is a ~39 kDa protein. Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube. Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).

1. Bourhis, E. et al. (2010) The Journal of biological chemistry 285, 9172-9. PMID: 20093360
2. Doubravska, L. et al. (2011) Cellular signalling 23, 837-48. PMID: 21244856
3. MacDonald, BT. et al. (2014) The Journal of biological chemistry 289, 18122-36. PMID: 24841207
4. Mihara, E. et al. (2016) eLife 5. PMID: 26902720
참고사항 For research use only.
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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