Human WNT3A Recombinant Protein (N-His) (HX182012)

Name: Human WNT3A Recombinant Protein (N-His)
Brand: abinScience
SKU: HX182012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HX182012
Description	Recombinant Human WNT3A Protein, N-His (HX182012) expressed in E. coli, spanning Ser19-Lys352. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P56704
Protein length	Ser19-Lys352
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	39.74 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Protein Wnt-3a, WNT3A
Background	Protein Wnt-3a (WNT3A) is a ~39 kDa protein. Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube. Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro). 1. Bourhis, E. et al. (2010) The Journal of biological chemistry 285, 9172-9. PMID: 20093360 2. Doubravska, L. et al. (2011) Cellular signalling 23, 837-48. PMID: 21244856 3. MacDonald, BT. et al. (2014) The Journal of biological chemistry 289, 18122-36. PMID: 24841207 4. Mihara, E. et al. (2016) eLife 5. PMID: 26902720
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human WNT3A Protein, N-His

References

HX182012-SDS-PAGE-1.jpg

Datasheet

Human WNT3A Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Human WNT3A Recombinant Protein (N-His) (HX182012)

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