Anti-WNT3A Polyclonal Antibody (HX182014)

Name: Anti-WNT3A Polyclonal Antibody
Brand: abinScience
SKU: HX182014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HX182014

Description

Anti-WNT3A Polyclonal Antibody (HX182014) is a rabbit polyclonal antibody detecting WNT3A in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Chicken, and Meleagris gallopavo.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human WNT3A (Ser19-Lys352).

Target

Protein Wnt-3a, WNT3A

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P56704

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Protein Wnt-3a (WNT3A) is a ~39 kDa protein. Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube. Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro).

1. Bourhis, E. et al. (2010) The Journal of biological chemistry 285, 9172-9. PMID: 20093360

2. Doubravska, L. et al. (2011) Cellular signalling 23, 837-48. PMID: 21244856

3. MacDonald, BT. et al. (2014) The Journal of biological chemistry 289, 18122-36. PMID: 24841207

4. Mihara, E. et al. (2016) eLife 5. PMID: 26902720

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-WNT3A polyclonal antibody at 1ug/mL on recombinant WNT3A (Catalog No. HX182012).

References

Datasheet

Anti-WNT3A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-WNT3A Polyclonal Antibody (HX182014)

Overview

Images

References

Datasheet

SDS

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