CHO Human CLDN18.2 Stable Cell Line (HX126828)

데이터시트 제품 공유 정보 복사

개요
이미지
참고 문헌
데이터시트

카탈로그 번호	HX126828
설명	CHO Human CLDN18.2 Stable Cell Line (HX126828) is a stable cell line expressing CLDN18.2. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
등록번호	P56856
응용 분야	FCM
숙주 종	CHO
종	Human
타겟	CLDN18.2
검출 방법	Colorimetric
샘플 유형	Plasma, Serum
분석 유형	Quantitative
범위	1.56 - 100 ng/mL
민감도	0.25 ng/mL
회수율	80-120%
특성	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
배송	2-8 ℃
안정성 및 저장	Frozen in liquid nitrogen.
사양	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
배경	Claudin-18 (CLDN18.2) is a ~27 kDa protein. Involved in alveolar fluid homeostasis via regulation of alveolar epithelial tight junction composition and therefore ion transport and solute permeability, potentially via downstream regulation of the actin cytoskeleton organization and beta-2-adrenergic signaling. Required for lung alveolarization and maintenance of the paracellular alveolar epithelial barrier. Acts to maintain epithelial progenitor cell proliferation and organ size, via regulation of YAP1 localization away from the nucleus and thereby restriction of YAP1 target gene transcription. Acts as a negative regulator of RANKL-induced osteoclast differentiation, potentially via relocation of TJP2/ZO-2 away from the nucleus, subsequently involved in bone resorption in response to calcium deficiency. Mediates the osteoprotective effects of estrogen, potentially via acting downstream of estrogen signaling independently of RANKL signaling pathways. CLDN18.2 is the therapeutic target of zolbetuximab (Vyloy).
참고사항	For research use only. This stable cell line expresses GFP as a reporter marker. GFP is expressed independently of the target protein and is not fused to the target protein.

Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CLDN18.2 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

참고 문헌

데이터시트