HEK293T Human CD158k/KIR3DL2 Stable Cell Line (HW633818)

카탈로그 번호	HW633818
설명	HEK293T Human CD158k/KIR3DL2 Stable Cell Line (HW633818) is a stable cell line expressing CD158k/KIR3DL2. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
등록번호	P43630
응용 분야	FCM
숙주 종	HEK293T
종	Human
타겟	CD158k/KIR3DL2
특성	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
안정성 및 저장	Frozen in liquid nitrogen.
사양	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
배경	Killer cell immunoglobulin-like receptor 3DL2 (CD158k/KIR3DL2) is a ~50 kDa protein. Receptor on natural killer (NK) cells and T cells for MHC class I molecules. Upon binding of peptide-free HLA-F open conformer, negatively regulates NK and T cell effector functions. Acts as a receptor on astrocytes for HLA-F. Through interaction with HLA-F, may protect motor neurons from astrocyte-induced toxicity. 1. Goodridge, JP. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 191, 3553-62. PMID: 24018270 2. Dulberger, CL. et al. (2017) Immunity 46, 1018-1029.e7. PMID: 28636952 3. Song, S. et al. (2016) Nature medicine 22, 397-403. PMID: 26928464

Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human KIR3DL2 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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