HEK293T Human CD16a/FCGR3A Stable Cell Line (HY386818)

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카탈로그 번호	HY386818
설명	HEK293T Human CD16a/FCGR3A Stable Cell Line (HY386818) is a stable cell line expressing CD16a/FCGR3A. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
등록번호	P08637
응용 분야	FCM
숙주 종	HEK293T
종	Human
타겟	CD16a/FCGR3A
특성	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
안정성 및 저장	Frozen in liquid nitrogen.
사양	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
배경	Low affinity immunoglobulin gamma Fc region receptor III-A (CD16a/FCGR3A) is a ~29 kDa protein. Receptor for the invariable Fc fragment of immunoglobulin gamma (IgG). Optimally activated upon binding of clustered antigen-IgG complexes displayed on cell surfaces, triggers lysis of antibody-coated cells, a process known as antibody-dependent cellular cytotoxicity (ADCC). Does not bind free monomeric IgG, thus avoiding inappropriate effector cell activation in the absence of antigenic trigger. Mediates IgG effector functions on natural killer (NK) cells. Binds antigen-IgG complexes generated upon infection and triggers NK cell-dependent cytokine production and degranulation to limit viral load and propagation. 1. Hezareh, M. et al. (2001) Journal of virology 75, 12161-8. PMID: 11711607 2. Ferrara, C. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 12669-74. PMID: 21768335 3. Mizushima, T. et al. (2011) Genes to cells : devoted to molecular & cellular mechanisms 16, 1071-80. PMID: 22023369 4. DiLillo, DJ. et al. (2014) Nature medicine 20, 143-51. PMID: 24412922 5. Lee, J. et al. (2015) Immunity 42, 431-42. PMID: 25786175 6. Jing, Y. et al. (2015) PloS one 10, e0121788. PMID: 25816339 7. Blázquez-Moreno, A. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E5645-E5654. PMID: 28652325 8. de Haas, M. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 2948-55. PMID: 8609432 9. Koene, HR. et al. (1997) Blood 90, 1109-14. PMID: 9242542

Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD16A monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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