HEK293T Human MICA Stable Cell Line (HS894818)

카탈로그 번호	HS894818
설명	HEK293T Human MICA Stable Cell Line (HS894818) is a stable cell line expressing MICA. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
등록번호	Q29983
응용 분야	FCM
숙주 종	HEK293T
종	Human
타겟	MICA
특성	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
안정성 및 저장	Frozen in liquid nitrogen.
사양	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
배경	MHC class I polypeptide-related sequence A (MICA) is a ~42 kDa protein. Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells. Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production. 1. Steinle, A. et al. (2001) Immunogenetics 53, 279-87. PMID: 11491531 2. Sutherland, CL. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 671-9. PMID: 11777960 3. Bauer, S. et al. (1999) Science (New York, N.Y.) 285, 727-9. PMID: 10426993

Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human MICA monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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