Anti-Human MICA Recombinant Antibody (16A8), FITC (HS894117)

Name: Anti-Human MICA Recombinant Antibody (16A8), FITC
Brand: abinScience
SKU: HS894117
Price: 163 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.	HS894117
Species reactivity	Human
Applications	FCM
Host species	Human
Isotype	IgG1, kappa
Clone ID	16A8
Conjugation	FITC
Clonality	Monoclonal
Target	MICA, MIC-A, MHC class I polypeptide-related sequence A, PERB11.1
Endotoxin level	Please contact with the lab for this information.
Associated products	Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), FITC
Accession	Q29983
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	MHC class I polypeptide-related sequence A (MICA) is a ~42 kDa protein. Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells. Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production. 1. Steinle, A. et al. (2001) Immunogenetics 53, 279-87. PMID: 11491531 2. Sutherland, CL. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 671-9. PMID: 11777960 3. Bauer, S. et al. (1999) Science (New York, N.Y.) 285, 727-9. PMID: 10426993
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow cytometry

Flow-cytometry FITC using anti-human MICA antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human MICA monoclonal antibody (Catalog HS894117) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry FITC using anti-human MICB antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human MICB monoclonal antibody (Catalog HS894117) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.