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| 카탈로그 번호 | JN185012 |
|---|---|
| 설명 |
IgM Scissors is a highly specific protease designed for controlled cleavage of immunoglobulin M (IgM). The enzyme selectively cleaves the μ heavy chain within the linker region between the CH2 and CH3 domains, enabling efficient fragmentation of IgM molecules while preserving antigen-binding activity. |
| 발현 시스템 | E. coli |
| 응용 분야 | Functional |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 형태 | Liquid |
| 저장 완충액 | PBS pH 7.4, 50% glycerol 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 참고사항 | For research use only |
| 생물학적 활성 | One unit (U) of IgM Scissors is defined as the amount of enzyme required to cleave 50 µg of IgM under standard conditions of 37°C for 3 hours. |
IgM Scissors is a specific IgM enzyme. It can digest human IgM at a specific site (..VPDQDT/AIRVFA..) below the CH2 domain in the heavy chain, producing F(ab')2 and Fc fragments
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