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| 카탈로그 번호 | HF827010 |
|---|---|
| 설명 |
InVivoMAb Anti-Human IFN α2/IFNA2 (Iv0001) [Iv0001] (HF827010) is a human monoclonal antibody detecting Interferon alpha-2 in ELISA, FCM, Neutralization. Suitable for Human.
Highlights
|
| 종 반응성 | Human |
| 응용 분야 | ELISA, FCM, WB, Neutralization |
| 숙주 종 | Human |
| 클론성 | Monoclonal |
| 이소타입 | IgG1 |
| 클론 ID | Iv0001 |
| 타겟 | Interferon alpha-2, IFN-alpha-2, Interferon alpha-A, LeIF A, IFNA2, IFN-Alpha, IFNA2A, IFNA2B, IFNA2C, Interferon alpha-1/13, IFN-alpha-1/13, Interferon alpha-D, LeIF D, IFNA1, IFNA13, IFN-α4, IFN-α5, IFN-α6, IFN-α7, IFN-α8, IFN-αl0, IFN-α14, IFN-α16, IFN-α17, IFN-α21 |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >95% as determined by SDS-PAGE. |
| 관련 제품 | pH 7.4 Dilution Buffer |
| 정제 | Protein A/G purified from cell culture supernatant. |
| 등록번호 | P01563 |
| 형태 | Liquid |
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 참고사항 | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
Detects Human IFN α2/IFNA2 in indirect ELISAs.
Flow-cytometry using anti-human IFN α2 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IFN α2 antibody monoclonal antibody (Catalog # HF827010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human IFN α2 antibody.SHSY-5Y cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IFN α2 antibody monoclonal antibody (Catalog # HF827010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for InVivoMAb Anti-Human IFN α2/IFNA2 (Iv0001).
Western blot analysis was performed using anti-IFNA2/IFN-alpha-2 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human IFNA2/IFN-alpha-2 (Catalog No: HF827012), 50ng
Lane 2: recombinant human IFNA2/IFN-alpha-2 (Catalog No: HF827012), 30ng
Lane 3: recombinant human IFNA2/IFN-alpha-2 (Catalog No: HF827012), 20ng
Lane 4: recombinant human IFNA2/IFN-alpha-2 (Catalog No: HF827012), 10ng
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