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PNGase F
PNGase F
PNGase F
PNGase F
PNGase F
PNGase F
가격(USD):
사양:
  • 15KU
  • 75KU
수량:
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  • 개요

  • 이미지

  • 참고 문헌

  • 데이터시트

  • SDS

개요
카탈로그 번호JN842012
발현 시스템E. coli
단백질 길이PNGase F is cloned from Elizabethkingia miricola and expressed in E.coli.
응용 분야Removal of high mannose N-glycans from glycoproteins
성질 Recombinant
내독소 수준 Please contact with the lab for this information.
순도 >95% as determined by SDS-PAGE.
형태 Liquid
저장 완충액 20 mM Tris-HCl, pH 7.5, 50mM NaCl, 5mM EDTA, 50% glycerol

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

배송 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름CAS: 83534-39-8
참고 문헌 1. Vilaj M, et al. Evaluation of different PNGase F enzymes in immunoglobulin G and total plasma N-glycans analysis. Glycobiology. 2021 Jan 9;31(1):2-7. [JN842012]
2. Huang J, et al. Highly Efficient Release of Glycopeptides from Hydrazide Beads by Hydroxylamine Assisted PNGase F Deglycosylation for N-Glycoproteome Analysis. Anal Chem. 2015 Oct 20;87(20):10199-204. [JN842012]
참고사항 For research use only.
이미지
  • PNGase F

    SDS-PAGE

    SDS-PAGE for PNGase F

  • PNGase F

    Bioactivity

    Deglycosylation of Human IgG1 Fc by PNGase F.

  • PNGase F

    Bioactivity

    Schematic diagram of PNGase F cleavage site.

참고 문헌
데이터시트
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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