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| 카탈로그 번호 | HB614012 |
|---|---|
| 설명 |
Recombinant Human ALOX15 Protein, N-His (HB614012) expressed in E. coli, spanning Lys170-Leu312. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | P16050 |
| 단백질 길이 | Lys170-Leu312 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 18.33 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | Arachidonate omega-6 lipoxygenase, Arachidonate 12-lipoxygenase, leukocyte-type, Hepoxilin A3 synthase Alox15, 12-LOX, 15-LOX-1, 15-LOX, Arachidonate 15-lipoxygenase, Polyunsaturated fatty acid lipoxygenase ALOX15, 12/15-lipoxygenase, Linoleate 13S-lipoxygenase, LOG15, ALOX15 |
| 배경 | Polyunsaturated fatty acid lipoxygenase ALOX15 is a ~74 kDa protein. Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. It inserts peroxyl groups at C12 or C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing both 12-hydroperoxyeicosatetraenoate/12-HPETE and 15-hydroperoxyeicosatetraenoate/15-HPETE. It may then act on 12-HPETE to produce hepoxilins, which may show pro-inflammatory properties. Can also peroxidize linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate/13-HPODE. May participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs)like resolvin D5 ((7S,17S)-diHPDHA) and (7S,14S)-diHPDHA, that actively down-regulate the immune response and have anti-aggregation properties with platelets. 1. Andersson, E. et al. (2006) Biochimica et biophysica acta 1761, 1498-505. PMID: 17052953 2. Sloane, DL. et al. (1991) Nature 354, 149-52. PMID: 1944593 3. Horn, T. et al. (2013) Redox biology 1, 566-77. PMID: 24282679 4. Teder, T. et al. (2014) Journal of lipid research 55, 2587-96. PMID: 25293588 5. Perry, SC. et al. (2020) Journal of lipid research 61, 1087-1103. PMID: 32404334 6. Kühn, H. et al. (1993) Biochimica et biophysica acta 1169, 80-9. PMID: 8334154 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human ALOX15 Protein, N-His
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