Anti-ALOX15 Polyclonal Antibody (HB614014)

Name: Anti-ALOX15 Polyclonal Antibody
Brand: abinScience
SKU: HB614014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HB614014

Description

Anti-ALOX15 Polyclonal Antibody (HB614014) is a rabbit polyclonal antibody detecting ALOX15 in ELISA, IHC, WB. Suitable for Human, Bovine, Pongo abelii, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ALOX15 (Lys170-Leu312).

Target

Arachidonate omega-6 lipoxygenase, Arachidonate 12-lipoxygenase, leukocyte-type, Hepoxilin A3 synthase Alox15, 12-LOX, 15-LOX-1, 15-LOX, Arachidonate 15-lipoxygenase, Polyunsaturated fatty acid lipoxygenase ALOX15, 12/15-lipoxygenase, Linoleate 13S-lipoxygenase, LOG15, ALOX15

Purification

Purified by antigen affinity column.

Accession

P16050

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Polyunsaturated fatty acid lipoxygenase ALOX15 is a ~74 kDa protein. Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. It inserts peroxyl groups at C12 or C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing both 12-hydroperoxyeicosatetraenoate/12-HPETE and 15-hydroperoxyeicosatetraenoate/15-HPETE. It may then act on 12-HPETE to produce hepoxilins, which may show pro-inflammatory properties. Can also peroxidize linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate/13-HPODE. May participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs)like resolvin D5 ((7S,17S)-diHPDHA) and (7S,14S)-diHPDHA, that actively down-regulate the immune response and have anti-aggregation properties with platelets.

1. Andersson, E. et al. (2006) Biochimica et biophysica acta 1761, 1498-505. PMID: 17052953

2. Sloane, DL. et al. (1991) Nature 354, 149-52. PMID: 1944593

3. Horn, T. et al. (2013) Redox biology 1, 566-77. PMID: 24282679

4. Teder, T. et al. (2014) Journal of lipid research 55, 2587-96. PMID: 25293588

5. Perry, SC. et al. (2020) Journal of lipid research 61, 1087-1103. PMID: 32404334

6. Kühn, H. et al. (1993) Biochimica et biophysica acta 1169, 80-9. PMID: 8334154

Note

For research use only.

Images

References

Datasheet

Anti-ALOX15 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-ALOX15 Polyclonal Antibody (HB614014)

Overview

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References

Datasheet

SDS

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