Human ALOX15B Recombinant Protein (N-His) (HT104032)

Name: Human ALOX15B Recombinant Protein (N-His)
Brand: abinScience
SKU: HT104032
Price: 211 USD
Availability: InStock

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100ug
1mg

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개요
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개요

카탈로그 번호	HT104032
설명	Recombinant Human ALOX15B Protein, N-His (HT104032) expressed in E. coli, spanning Met1-Gly121. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
발현 시스템	E. coli
등록번호	O15296
단백질 길이	Met1-Gly121
응용 분야	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
종	Homo sapiens (Human)
성질	Recombinant
내독소 수준	Please contact with the lab for this information.
순도	>90% as determined by SDS-PAGE.
예측 분자량	15.36 kDa
형태	Lyophilized
저장 완충액	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
재구성	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
배송	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름	15-LOX-2, 15-LOX-B, 15-lipoxygenase 2, ALOX15B, Arachidonate 15-lipoxygenase B, Arachidonate 15-lipoxygenase type II, EC:1.13.11.-, EC:1.13.11.33, Linoleate 13-lipoxygenase 15-LOb, Polyunsaturated fatty acid lipoxygenase ALOX15B
배경	Polyunsaturated fatty acid lipoxygenase ALOX15B is a ~75 kDa protein. Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids (PUFAs) generating a spectrum of bioactive lipid mediators (Probable). It inserts peroxyl groups at C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing (15S)-hydroperoxyeicosatetraenoate/(15S)-HPETE (Probable). Also peroxidizes linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate/13-HPODE (Probable). Oxygenates arachidonyl derivatives such as 2-arachidonoylglycerol (2-AG) leading to the production and extracellular release of 15-hydroxyeicosatetraenoyl glycerol (15-HETE-G) that acts as a peroxisome proliferator-activated receptor alpha agonist. Has the ability to efficiently class-switch ALOX5 pro-inflammatory mediators into anti-inflammatory intermediates. 1. Kilty, I. et al. (1999) European journal of biochemistry 266, 83-93. PMID: 10542053 2. Jisaka, M. et al. (2000) The Journal of biological chemistry 275, 1287-93. PMID: 10625675 3. Bhatia, B. et al. (2003) The Journal of biological chemistry 278, 25091-100. PMID: 12704195 4. Prusakiewicz, JJ. et al. (2007) Archives of biochemistry and biophysics 464, 260-8. PMID: 17493578 5. Turman, MV. et al. (2008) Biochemistry 47, 3917-25. PMID: 18311922 6. Horn, T. et al. (2013) Redox biology 1, 566-77. PMID: 24282679 7. Kobe, MJ. et al. (2014) The Journal of biological chemistry 289, 8562-9. PMID: 24497644 8. Perry, SC. et al. (2020) Journal of lipid research 61, 1087-1103. PMID: 32404334 9. Brash, AR. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 6148-52. PMID: 9177185
참고사항	For research use only

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Human ALOX15B Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

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Volume

Molecular Weight *

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Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Human ALOX15B Recombinant Protein (N-His) (HT104032)

개요

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